Serum MicroRNAs as Biomarkers of Sepsis and Resuscitation

There is a lack of biomarkers sepsis and the resuscitation status. Our objective was to prove that serum expression certain microribonucleic acids (miRNAs) differentially regulated in sensitive different regimes. Anesthetized pigs (Sus scrofa domesticus) received no treatment (n = 15) or intravenous live E. coli 24). The septic animals 0.9% saline at 4 mL/kg/h 8) (low group (LoR)) 10–17 (high (HiR)) 8 each group). Blood samples were obtained end experiment for measurement seven miRNAs (RT-qPCR, Qiagen, Hilden, Germany). miR-146a-5p miR-34a-5p increased significantly group, lower HiR than LoR group. toll-like receptor signaling pathway involving 22 target proteins (adjusted p 3.87 × 10−4) by these two microRNAs (KEGG). Highly significant (p value 2.22 10−16) protein–protein interactions (STRING) revealed hits. MiR-146a-5p identified as sepsis, miRNA146a-5p seemed be biomarker intensity resuscitation.